Site specific protein O-glucosylation with bacterial toxins.
نویسندگان
چکیده
Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.
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ورودعنوان ژورنال:
- Chemical communications
دوره 52 88 شماره
صفحات -
تاریخ انتشار 2016